Anti-GABA(A)R, Alpha2 [N399/19R] in Western Blot (Rat)

Hybridoma

Data Submitted By: Uwe Rudolph
Lab Name: Uwe Rudolph, Harvard Medical School
Submission Date: February 06, 2024
Publication Date: February 06, 2024 (modified March 18, 2024)
Abstract: Experimental results for Anti-GABA(A)R, Alpha2 [N399/19R] (hybridoma-derived) in a western blot assay with a rat sample.

Immunoblot against crude membranes from adult rat brain (RBM) and wild-type (WT) and GABA-A-R-Alpha2 knockout (KO) mouse brains probed with N399/19 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Uwe Rudolph (Harvard Medical School). — Caption: Immunoblot against crude membranes from adult rat brain (RBM) and wild-type (WT) and GABA-A-R-Alpha2 knockout (KO) mouse brains probed with N399/19 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Uwe Rudolph (Harvard Medical School).
Image attribution: Uwe Rudolph, Harvard Medical School

Any further use of this image requires permission from the lab who submitted the data.

Related Antibody: Anti-GABA(A)R, Alpha2 [N399/19R]
Addgene #190894

Materials & Methods

Sample

Species: Rat
Cell / Tissue Type: Brain

Primary Antibody

Name: Anti-GABA(A)R, Alpha2 [N399/19R]
Species: Mouse
Gene / Target: Gabra2

Additional Information

Additional Information: NeuroMab hybridoma clone datasheet (Link opens in a new window)

Results

Result: Pass (The antibody worked under these conditions)
Knockout Validation: Yes