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Anti-BAF53b [N332B/15R] in Immunohistochemistry (Rat)

Addgene #184200

Data Submitted By
James Trimmer
Lab Name
James Trimmer, UC Davis
Submission Date
February 06, 2024
Publication Date
February 06, 2024 (modified February 06, 2024)
Abstract
Experimental results for Anti-BAF53b [N332B/15R] (Addgene Antibody 184200) in an immunohistochemistry assay with a rat sample.
Caption: Free-floating rat brain sections were fixed and stained with purified recombinant Anti-BAF53b [N332B/15R]. The staining patterns from antibody produced at Addgene (lot p7685) were compared to UC Davis-derived antibody (TC Supe).
Image attribution: James Trimmer, UC Davis

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used
Anti-BAF53b [N332B/15R]
Addgene #184200
mix of biotinylated goat anti-mouse IgG1, goat anti-mouse IgG2a, goat anti-mouse IgG2b
Jackson Labs #115-065-205, 115-065-206, 115-065-207

Materials & Methods

Sample

Species
Rat
Cell / Tissue Type
Brain

Fixative

Fixative
Formaldehyde/Paraformaldehyde

Permeabilization / Delipidation

Agent
PBS containing 1:2000 avidin and 0.5% Triton X-100
Time
45 min
Temperature
Room Temperature

Blocking

Agent
Serum
Concentration
0.5%
Time
45 min
Temperature
Room Temperature

Primary Antibody

Name
Anti-BAF53b [N332B/15R]
Source
Addgene
Catalog Number
184200
Species
Mouse
Gene / Target
Actl6b
Concentration
1 µg/mL
Time
overnight
Temperature
4 °C
View in Addgene Catalog

Secondary Antibody

Name
mix of biotinylated goat anti-mouse IgG1, goat anti-mouse IgG2a, goat anti-mouse IgG2b
Source
Jackson Labs
Catalog Number
115-065-205, 115-065-206, 115-065-207
Concentration
0.5 µg/mL
Time
1 hour
Temperature
Room Temperature

Additional Information

Additional Information

Tissue type: Free-floating brain sections

Antigen-retrieval: No

NeuroMab hybridoma clone datasheet (Link opens in a new window)

Protocol
Trimmer lab immunohistochemistry protocol (Link opens in a new window)

Results

Result
Pass (The antibody worked under these conditions)