pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 in Mouse (AAV1)
Addgene #98928-AAV1
- Data Submitted By
- Sindhuja Baskar and David Margolis
- Submission Date
- October 17, 2019
- Publication Date
- October 17, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Image attribution: Branden Sanabria
Any further use of this image requires permission from the lab who submitted the data.
- Vectors Used
-
pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40
Addgene #98928-AAV1 -
pENN.AAV.CamKII 0.4.Cre.SV40
Addgene #105558-AAV9
Virus & Injection
Virus 1
- Virus Name
- pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40
- Serotype
- AAV1
- Source
- Addgene
- Catalog Number
- 98928-AAV1
- Promoter
- CAG
- Cargo Type
- Control
- Injection Titer / Dose
- 5.00E+12 GC/mL
- Injection Volume
- 210 nL
- Injection Rate
- 70 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Primary motor cortex, NeuroNames ID: 1910, Primary somatosensory cortex NeuroNames ID: 1350
Virus 2
- Virus Name
- pENN.AAV.CamKII 0.4.Cre.SV40
- Serotype
- AAV9
- Source
- Addgene
- Catalog Number
- 105558-AAV9
- Promoter
- CaMKII
- Cargo Type
- Recombinase
- Injection Titer / Dose
- 5.00E+12 GC/mL
- Injection Volume
- 210 nL
- Injection Rate
- 70 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Primary motor cortex, NeuroNames ID: 1910, Primary somatosensory cortex NeuroNames ID: 1350, Rhinencephalon, NeuroNames ID: 2078
- Additional Details
- Primary Motor or Primary Somatosensory Cortex; Forebrain: Rhinencephalon (paleocortex).
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J
- Source
- Jackson Labs
- Stock Number
- JAX 000664
- Age at Injection
- 45-60 days old
- Time After Injection
- 4 weeks
- Detection Method
- Immunohistochemistry
- Assay & Results
- Pyramidal cells in cortex, expressing through the terminals into dorsal striatum.