pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 in Mouse (AAV1)
Addgene #98928-AAV1

Confocal tiled stack of Ruby2 labeled cortical pyramidal cells projecting from cortex to dorsal striatum in mouse coronal slice.

Caption: Confocal tiled stack of Ruby2 labeled cortical pyramidal cells projecting from cortex to dorsal striatum in mouse coronal slice.

Image attribution: Branden Sanabria

Viruses & Injection

Primary Virus
Promoter
CAG
Injected Titer
5.00E+12 GC/ml
Injected Volume
210nL
Injection Rate
0. 07ul/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Primary Motor or Primary Somatosensory cortex
View in Addgene Catalog
Secondary Virus
pENN.AAV.CamKII 0.4.Cre.SV40 (AAV9)
Promoter
CamKII
Injected Titer
5x10^12vg/mL GC/ml
Injected Volume
210nL
Injection Rate
0. 07uL/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Rhinencephalon (paleocortex)
Injection Site Comments
Primary Motor or Primary Somatosensory Cortex
View in Addgene Catalog

Subject

Species / Strain
Mouse mouse C57/BL6J
Stock Number
JAX 000664
Age at Injection
45-60

Data Collection

Time After Injection
4 weeks
Detection Method
Immunohistochemistry
Expression Site
Pyramidal cells in cortex, expressing through the terminals into dorsal striatum