pAAV-Ef1a-DIO hChR2(E123A)-EYFP in Rat (AAV9)
Addgene #35507-AAV9
- Data Submitted By
- Todd Nentwig and L. Judson Chandler
- Submission Date
- August 02, 2019
- Publication Date
- August 02, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
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Any further use of this image requires permission from the lab who submitted the data.
- Vectors Used
-
pAAV-Ef1a-DIO hChR2(E123A)-EYFP
Addgene #35507-AAV9 -
pENN.AAV.hSyn.Cre.WPRE.hGH
Addgene #105553-AAVrg
Virus & Injection
Virus 1
- Virus Name
- pAAV-Ef1a-DIO hChR2(E123A)-EYFP
- Serotype
- AAV9
- Source
- Addgene
- Catalog Number
- 35507-AAV9
- Promoter
- EF1a
- Cargo Type
- Optogenetics
- Injection Titer / Dose
- 2.50E+13 GC/mL
- Injection Volume
- 400 nL
- Injection Rate
- 60 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Prelimbic area, NeuroNames ID: 1078
Virus 2
- Virus Name
- pENN.AAV.hSyn.Cre.WPRE.hGH
- Serotype
- AAV Retrograde
- Source
- Addgene
- Catalog Number
- 105553-AAVrg
- Promoter
- Synapsin
- Injection Titer / Dose
- 1.20E+13 GC/mL
- Injection Volume
- 200 nL
- Injection Rate
- 60 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Epithalamus, NeuroNames ID : 292
- Additional Details
Other Details
- Species
- Rat
- Strain or Cell Line
- Long-Evans
- Source
- Not provided
- Age at Injection
- ~3 months old
- Time After Injection
- 8 weeks
- Detection Method
- Immunohistochemistry
- Assay & Results
- Prelimbic Cortex neurons projecting to medial dorsal thalamic/habenular area. Prelimbic cortex, corticothalamic projection neurons.
- Target Cell Transduction
- Prelimbic cortex neurons projecting to thalamic/habenular areas are shown expressing Ef1a-DIO-hChR2(E123A)-EYFP.
- Functional Testing
- Pilot testing virus expression and optic fiber placement. No light was used in these experiments to activate the opsin.
- Other Information
- The only inflammation/glial scarring that was observed was a result of optic fiber implantation above the region of viral expression. Dexamethasone treatment prior to and for ~3 days after surgery may help to reduce inflammation around optic fiber site.