pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA in Mouse (AAV1)
Addgene #50943-AAV1

Caption: None provided

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
1.00E+10 GC/ml
Injected Volume
4 μl (2 μl per hemisphere)
Injection Rate
Injections were done by hand
Injection Site
Cerebrospinal fluid (CSF): Intracerebroventricular (ICV)
Brain Region
We target the ventricles for widepsread transduction
Injection Site Comments
Neurons were targeted
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Subject

Species / Strain
Mouse B6SJL F1 hybrid
Stock Number
100012
Age at Injection
P0 (first 24 hrs after birth)

Data Collection

Time After Injection
1 month to 6 months
Detection Method
Immunohistochemistry, Direct fluorescence
Expression Site
Neurons in forebrain- cortex, hippocampus, some mid brain
Genetic Tool Outcome
The GCaMP6 activated spontaneously in some neurons, suggesting it worked effectively. The mRuby was visible in cell bodies and neurites, marking transduced cells
Other Information
No evidence of toxicity or other adverse effects. Expression persisted through life of animal, up to at least 16 months