AAV pmSyn1-EBFP-Cre in Mouse (AAV1)
Addgene #51507-AAV1

Imaged with alexaFluor 647 secondary against anti-HA antibody. LSM700 with 10x objective.

Caption: Imaged with alexaFluor 647 secondary against anti-HA antibody. LSM700 with 10x objective.

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
4.50E+12 GC/ml
Injected Volume
600 nl
Injection Rate
200 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Hippocampus
Injection Site Comments
Injecting into ventral hippocampus, targeting all cell types. Mouse used was RiboTag-HA mouse, so Cre was used to facilitate HA expression in all hippocampal neurons
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
JAX: 011029
Age at Injection
3 months

Data Collection

Time After Injection
4 weeks
Detection Method
Immunohistochemistry
Expression Site
All cells in the ventral hippocampus
Genetic Tool Outcome
No tools were used
Other Information
No toxicity, no inflammation
Tips & Comments
The virus for this experiment worked very well