AAV pmSyn1-EBFP-Cre in Rat (AAV Retrograde)
Addgene #51507-AAVrg

anti-GFP (tag for rgAAV-cre) in green, anti-Substance P in red, bright field/fluorescent microscope, 1.25x objective

Caption: anti-GFP (tag for rgAAV-cre) in green, anti-Substance P in red, bright field/fluorescent microscope, 1.25x objective

Image attribution: Mitchell Farell

anti-mCherry (tag for DIO-hM4D(Gi) AAV) in red, bright field/fluorescent microscope, 5x objective

Caption: anti-mCherry (tag for DIO-hM4D(Gi) AAV) in red, bright field/fluorescent microscope, 5x objective

Image attribution: Mitchell Farrell

Viruses & Injection

Primary Virus
Promoter
Syn
Injected Titer
6.00E+12 GC/ml
Injected Volume
300 nl
Injection Rate
600 nl/min
Injection Site
Brain parenchyma
Brain Region
Ventral pallidum
Injection Site Comments
Targeting neurons in Ventral Pallidum with AAVrg and Nucleus Accumbens cells that project to VP with DIO-hM4DGi
View in Addgene Catalog
Secondary Virus
pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV2)
Promoter
Syn
Injected Titer
5.00E+12 GC/ml
Injected Volume
300 nl
Injection Rate
600 nl/min
Injection Site
Brain parenchyma
Brain Region
Nucleus accumbens
Injection Site Comments
Targeting neurons in nucleus accumbens projecting to ventral pallidum. Injected 15 min prior to previous injection
View in Addgene Catalog

Subject

Species / Strain
Rat Wild-type Long Evans
Stock Number
Envigo
Age at Injection
postnatal day 60-80

Data Collection

Time After Injection
3 weeks
Detection Method
Immunofluorescence for mCherry and GFP
Expression Site
AAVrg-cre was visualized in VP cells expressing synapsin. DIO-hM4D(Gi) was visualized in cells of NAc that project to VP
Other Information
No toxicity or inflammation seen
Tips & Comments
We waited 5 minutes before removing the injection pipette from the injection site