AAV pmSyn1-EBFP-Cre in Mouse (AAV Retrograde)
Addgene #51507-AAVrg

Imaged with 649 nm (used AlexaFluor 647 secondary antibody for anti-HA primary), and 405 nm (DAPI) lasers on a confocal LSM 700 microscope

Caption: Imaged with 649 nm (used AlexaFluor 647 secondary antibody for anti-HA primary), and 405 nm (DAPI) lasers on a confocal LSM 700 microscope

Image attribution: Henry Hallock

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
1.10E+13 GC/ml
Injected Volume
600 nl
Injection Rate
200 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Injected into prelimbic sub-region of the medial prefrontal cortex
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
JAX: 011029
Age at Injection
3 months

Data Collection

Time After Injection
4 weeks
Detection Method
Immunohistochemistry
Expression Site
Ventral CA1 neurons with projections to the PrL
Genetic Tool Outcome
We used a RiboTag mouse to drive HA protein expression with Cre, and this worked very well
Other Information
No toxicity/inflammation
Tips & Comments
The retro-Cre drove HA production very well in this mouse