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Anti-Integrin alpha M [IPI-CBRM1/29] in Flow Cytometry (Human)

Addgene #213667

Data Submitted By
IPI Staff
Lab Name
Institute for Protein Innovation (IPI)
Submission Date
March 20, 2024
Publication Date
March 25, 2024 (modified March 25, 2024)
Abstract
Experimental results for Anti-Integrin alpha M [IPI-CBRM1/29] (Addgene Antibody 213667) in a flow cytometry assay with a human sample.
Caption: Flow cytometry data showing positive labeling by Anti-Integrin alpha M [IPI-CBRM1/29] antibody (#213667) in human CD8+ T cells, B cells, and NK cells. Human peripheral blood mononuclear cells (PBMCs) were isolated from whole blood samples and labeled with Anti-Integrin alpha M [IPI-CBRM1/29] antibody conjugated with BroadPharma Fluor 488 NHS Ester (BP488). Control samples were labeled with BP488-conjugated rabbit isotype control (Cell Signaling Technologies #3900S). The cells were also labeled with cell type markers to distinguish leukocytes (CD45), T cells (CD3), cytotoxic T cells (CD3/CD8), helper T cells (CD3/CD4), B cells (CD19), and NK cells (CD49). After labeling and washing, flow cytometry experiments were performed on a CytoFLEX flow cytometer.
Image attribution: Institute for Protein Innovation (IPI)

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibody Used
Anti-Integrin alpha M [IPI-CBRM1/29]
Addgene #213667

Materials & Methods

Sample

Species
Human
Cell / Tissue Type
Peripheral blood mononuclear cells (PBMC)

Blocking

Agent
1% BSA with Fc blocker
Time
20 min
Temperature
4 ˚C

Primary Antibody

Name
Anti-Integrin alpha M [IPI-CBRM1/29]
Source
Addgene
Catalog Number
213667
Species
Rabbit
Gene / Target
ITGAM
Concentration
10 µg/mL
Conjugate
BroadPharma Fluor 488 NHS Ester (BP488)
Time
30 min
Temperature
4 ˚C
View in Addgene Catalog

Additional Information

Additional Information

Human PBMCs were isolated from whole blood sample using a Cell Preparation Tube (CPT) with sodium heparin and resuspended in Dulbecco's Phosphate Buffered Saline (DPBS)-based blocking buffer.

Detection: Flow cytometry experiments were performed on a CytoFLEX flow cytometer. The cells were also labeled with cell type markers to distinguish leukocytes (CD45), T cells (CD3), cytotoxic T cells (CD3/CD8), helper T cells (CD3/CD4), B cells (CD19), and NK cells (CD49). Control samples were labeled with BP488-conjugated rabbit isotype control (Cell Signaling Technologies #3900S).

Results

Result
Pass (The antibody worked under these conditions)