Anti-Integrin alpha L [IPI-M17/4] in Flow Cytometry (Mouse)
Addgene #213673
- Data Submitted By
- IPI Staff
- Lab Name
- Institute for Protein Innovation (IPI)
- Submission Date
- March 25, 2024
- Publication Date
- March 25, 2024 (modified March 27, 2024)
- Abstract
- Experimental results for Anti-Integrin alpha L [IPI-M17/4] (Addgene Antibody 213673) in a flow cytometry assay using Expi CHO cells transiently transfected with a chimeric receptor consisting of the murine αL and human β2 chains.
Image attribution: Institute for Protein Innovation (IPI)
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibody Used
-
Anti-Integrin alpha L [IPI-M17/4]
Addgene #213673
Materials & Methods
Sample
- Species
- Mouse
- Cell / Tissue Type
- Expi CHO cells
- Additional Information
- Expi CHO cells were transiently transfected with B7H3 (Control) or a chimeric receptor consisting of the murine αL and human β2 chains.
Primary Antibody
- Name
- Anti-Integrin alpha L [IPI-M17/4]
- Source
- Addgene
- Catalog Number
- 213673
- Species
- Rat
- Gene / Target
- Itgal
- Concentration
- 10 µg/mL
- Time
- 30 min
- Temperature
- 4 ˚C
Additional Information
- Additional Information
This data compares labeling by the rabbit chimera recombinant version of the IPI-M17/4 antibody (Addgene #213663) and the rat recombinant version (Addgene #213673) to a hybridoma version (eBioscience, Catalog number 14-0111-85). Use of the hybridoma antibody was first reported in (Sanchez-Madrid et al. 1982).
Results
- Result
- Pass (The antibody worked under these conditions)