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pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 in Mouse (AAV9)

Addgene #108912-AAV9

Data Submitted By
Julie Haas
Submission Date
July 17, 2020
Publication Date
July 17, 2020 (modified May 16, 2023)
Abstract
Not provided.
Caption: DAPI (magenta) and mRuby3 (red) expression in thalamic reticular nucleus. Magnification 40x.
Image attribution: Atehsa Sahagun, Kevin Bender

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3
Addgene #108912-AAV9

Virus & Injection

Virus

Virus Name
pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3
Serotype
AAV9
Source
Addgene
Catalog Number
108912-AAV9
Promoter
CAG
Cargo Type
Optogenetics
Injection Titer / Dose
1.00E+13 GC/ml
Injection Volume
two 200 nl injections
Injection Rate
100 nl/min
Injector Material
Not provided
Injection Site / Route
Reticular nucleus of the thalamus NeuroNames ID: 365
Additional Details
Thalamic reticular nucleus - GABAergic neurons; Forebrain: Thalamus; TRN neurons.
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
PV-cre heterozygote
Source
Not provided
Age at Injection
P28
Time After Injection
3 weeks
Detection Method
Direct fluorescence, Whole-cell patch clamp
Assay & Results
Not provided
Functional Testing
ChroME, 1040 nm light used to drive spiking
Other Information
At 9 weeks* of expression, mRuby was widely diffuse, not nuclear. *Opportunity provided by covid-19