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pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 in Mouse (AAV1)

Addgene #100848-AAV1

Data Submitted By
Ruxandra Barzan and Dirk Jancke
Submission Date
July 03, 2020
Publication Date
July 03, 2020 (modified May 16, 2023)
Abstract
Not provided
Caption: RCaMP expression (red) in the mouse primary visual cortex. Image taken with a confocal microscope, 20x objective. Scale bar = 100 μm
Image attribution: Zohre Azimi

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV.Syn.NES.jRCaMP1a.WPRE.SV40
Addgene #100848-AAV1

Virus & Injection

Virus

Virus Name
pAAV.Syn.NES.jRCaMP1a.WPRE.SV40
Serotype
AAV1
Source
Addgene
Catalog Number
100848-AAV1
Promoter
Synapsin
Cargo Type
Biosensor
Injection Titer / Dose
1.00E+13 GC/ml
Injection Volume
Two 0.5 μl injections
Injection Rate
0.02 μl/min
Injector Material
Not provided
Injection Site / Route
Visual cortex, NeuroNames ID: 2102
Additional Details
Injections at two different sites within the primary visual cortex, each in 3 steps at depths between 600 and 200 μm below the brain surface. Break for 10 min between injection steps; Primary visual cortex
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
mouse C57BL/6J, ePetCre
Source
Not provided
Age at Injection
2 months
Time After Injection
2 weeks
Detection Method
Direct fluorescence, wide-field calcium imaging
Assay & Results
Not Provided
Functional Testing
Good visually evoked calcium responses (see Azimi et al. , 2020; doi: 10. 7554/eLife. 53552).
Other Information
No toxicity or inflammation seen.