pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 in Mouse (AAV1)
Addgene #100848-AAV1

RCaMP expression (red) in the mouse primary visual cortex. Image taken with a confocal microscope, 20x objective. Scale bar = 100 μm

Caption: RCaMP expression (red) in the mouse primary visual cortex. Image taken with a confocal microscope, 20x objective. Scale bar = 100 μm

Image attribution: Zohre Azimi

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
1.00E+13 GC/ml
Injected Volume
Two 0.5 μl injections
Injection Rate
0.02 μl/min
Injection Site
Brain parenchyma
Brain Region
Primary visual cortex
Injection Site Comments
Injections at two different sites within the primary visual cortex, each in 3 steps at depths between 600 and 200 μm below the brain surface. Break for 10 min between injection steps
View in Addgene Catalog

Subject

Species / Strain
Mouse mouse C57BL/6J, ePetCre
Age at Injection
2 months

Data Collection

Time After Injection
2 weeks
Detection Method
Direct fluorescence, wide-field calcium imaging
Genetic Tool Outcome
Good visually evoked calcium responses (see Azimi et al. , 2020; doi: 10. 7554/eLife. 53552)
Other Information
No toxicity or inflammation seen