pGP-AAV-syn-jGCaMP7f-WPRE in Mouse (AAV Retrograde)
Addgene #104488-AAVrg

Midbrain tegmentum area neurons expressing retro-AAV-jGCaMP7f. Immunostaining was used to identify the following targets—GFP: GcaMP7f (green); TH: Dopamine neurons (blue). A Zeiss AxioImager M2 upright widefield fluorescence/differential interference contrast microscope (5x objective) was used to obtain the image.

Caption: Midbrain tegmentum area neurons expressing retro-AAV-jGCaMP7f. Immunostaining was used to identify the following targets—GFP: GcaMP7f (green); TH: Dopamine neurons (blue). A Zeiss AxioImager M2 upright widefield fluorescence/differential interference contrast microscope (5x objective) was used to obtain the image.

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
7.00E+12 GC/ml
Injected Volume
400nl
Injection Rate
100 nl/min
Injection Site
Brain parenchyma
Brain Region
Midbrain: Tegmentum: Substantia nigra
Injection Site Comments
Lateral SNR
View in Addgene Catalog

Subject

Species / Strain
Mouse mouse C57BL/6J6J
Age at Injection
8 weeks

Data Collection

Time After Injection
3 weeks
Detection Method
Immunohistochemistry
Expression Site
Midbrain Tegmentum area, including VTA and SNR neurons
Genetic Tool Outcome
It worked as expected
Other Information
This virus has low toxicity and works really well for measuring neuronal activity