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pAAV.GfaABC1D.PI.Lck-GFP.SV40 in Rat (AAV5)

Addgene #105598-AAV5

Data Submitted By
Todd Nentwig and L. Judson Chandler
Submission Date
January 05, 2020
Publication Date
January 05, 2020 (modified May 16, 2023)
Abstract
Not provided
Caption: Rat Lateral Habenula; 10x mag. Astrocytes labeled using GfaABC1D.PI.Lck-GFP virus (green). Synapsin and Glutamate Transporter 1 (GLT1) are labeled with red and cyan staining, respectively.
Image attribution: Anna Kruyer

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV.GfaABC1D.PI.Lck-GFP.SV40
Addgene #105598-AAV5

Virus & Injection

Virus

Virus Name
pAAV.GfaABC1D.PI.Lck-GFP.SV40
Serotype
AAV5
Source
Addgene
Catalog Number
105598-AAV5
Promoter
GFAP
Cargo Type
Control
Injection Titer / Dose
7.00E+12 GC/ml
Injection Volume
200 nl
Injection Rate
1 nl/sec
Injector Material
Not provided
Injection Site / Route
Lateral Habenula, NeuroNames ID: 295
Additional Details
Lateral habenula; Lateral Habenula Astrocytes
View in Addgene Catalog

Other Details

Species
Rat
Strain or Cell Line
Sprague-Dawley
Age at Injection
~150 days
Time After Injection
4 weeks
Detection Method
Direct fluorescence
Tips & Comments
Direct fluorescence was sufficient to visualize site/spread of virus expression but immuno-amplification is likely necessary if performing high magnification, quantitative analysis of astrocyte-neuron interactions