AAV pCAG-FLEX-EGFP-WPRE in Mouse (AAV1)
Addgene #51502-AAV1

Z-stack taken in vivo 58 um max projection of layer 2/3 neurons.

Caption: Z-stack taken in vivo 58 um max projection of layer 2/3 neurons.

Viruses & Injection

Primary Virus
Promoter
CAG
Injected Titer
1.00E+13 GC/ml
Injected Volume
two 100 nl injections (200 nl total)
Injection Rate
36.8 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Binocular Visual Cortex
View in Addgene Catalog
Secondary Virus
pENN.AAV.CamKII 0.4.Cre.SV40 (AAV1)
Promoter
CaMKII
Injected Titer
1.00E+13 GC/ml
Injected Volume
100 nl
Injection Rate
36.8 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Excitatory cell types, same surgery
View in Addgene Catalog

Subject

Species / Strain
Mouse C57BL/6J
Age at Injection
P49

Data Collection

Time After Injection
2 weeks
Detection Method
Direct fluorescence
Expression Site
Binocular Visual Cortex, did not confirm cell identity by secondary staining
Genetic Tool Outcome
Spiny projection neurons successfully labeled
Other Information
Poor visibility in immediate injection site when using in vivo two-photon imaging
Tips & Comments
Less/diluted Cre could have been used