pAAV.Syn.NES-jRGECO1a.WPRE.SV40 in Mouse (AAV1)
Addgene #100854-AAV1

Confocal image of mouse striatum and cortex showing jRGECO labeling.

Caption: Confocal image of mouse striatum and cortex showing jRGECO labeling.

Viruses & Injection

Primary Virus
Promoter
Syn
Injected Titer
2.40E+13 GC/ml
Injected Volume
~210-420 nl
Injection Rate
0.042-0.084 μl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Basal Ganglia: Striatum (Putamen or caudate)
Injection Site Comments
Injection site: whisker M1
View in Addgene Catalog
Secondary Virus
pGP-AAV-syn-jGCaMP7f-WPRE (AAV1)
Promoter
Syn
Injected Titer
2.20E+13 GC/ml
Injected Volume
~210-420 nl
Injection Rate
0.042-0.084 μl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Basal Ganglia: Striatum (Putamen or caudate)
Injection Site Comments
Injection site: whisker M1 Where: primary motor cortex (M1), primary somatosensory cortex (S1), striatum, posterior medial nucleus of the thalamus (POm)
View in Addgene Catalog
Other Virus
pAAV-CaMKIIa-hChR2(H134R)-EYFP
Promoter
CaMKIIa
Injected Titer
1.00E+13 GC/ml
Injected Volume
~210 nl
Injection Rate
0.042 μl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Thalamus
Injection Site Comments
Primary motor cortex (M1), primary somatosensory cortex (S1), posterior medial nucleus of the thalamus (POm)

Subject

Species / Strain
Mouse C57Bl/6J, B6. Tg(Gpr26-cre)KO250
Stock Number
Jax mouse Stock No: 000664
Age at Injection
49-78 days

Data Collection

Time After Injection
3 weeks
Detection Method
Fiber Photometry