pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 in Mouse (AAV1)
Addgene #98928-AAV1
Caption: Confocal tiled stack of Ruby2 labeled cortical pyramidal cells projecting from cortex to dorsal striatum in mouse coronal slice.
Image attribution: Branden Sanabria
Viruses & Injection
- Primary Virus
- Promoter
- CAG
- Injected Titer
- 5.00E+12 GC/ml
- Injected Volume
- 210nL
- Injection Rate
- 0. 07ul/min
- Injection Site
- Brain parenchyma
- Brain Region
- Forebrain: Cerebral cortex (neocortex)
- Injection Site Comments
- Primary Motor or Primary Somatosensory cortex
- Secondary Virus
- pENN.AAV.CamKII 0.4.Cre.SV40 (AAV9)
- Promoter
- CamKII
- Injected Titer
- 5x10^12vg/mL GC/ml
- Injected Volume
- 210nL
- Injection Rate
- 0. 07uL/min
- Injection Site
- Brain parenchyma
- Brain Region
- Forebrain: Rhinencephalon (paleocortex)
- Injection Site Comments
- Primary Motor or Primary Somatosensory Cortex
Subject
- Species / Strain
- Mouse mouse C57/BL6J
- Stock Number
- JAX 000664
- Age at Injection
- 45-60
Data Collection
- Time After Injection
- 4 weeks
- Detection Method
- Immunohistochemistry
- Expression Site
- Pyramidal cells in cortex, expressing through the terminals into dorsal striatum