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pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 in Mouse (AAV9)
Addgene #100842-AAV9

GCaMP expressing neurons are visualized by anti-GFP staining (green). Coronal section. Blue, DAPI, 3V, third ventricle. Scale bar, 100 um. — Caption: GCaMP expressing neurons are visualized by anti-GFP staining (green). Coronal section. Blue, DAPI, 3V, third ventricle. Scale bar, 100 um.

Data Submitted By

Kengo Inada and Kazunari Miyamichi

Submission date: August 11, 2019

Any further use of this image requires the permission of the lab.

This scientist responded to a specific request for user data and was compensated for time and input.

Vectors Used

pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 #100842-AAV9 deposited by Douglas Kim, GENIE Project

Virus & Injection

Primary Virus: pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 (AAV9)
Promoter: CAG
Injected Titer: 1.90E+13 GC/ml
Injected Volume: 400 nl
Injection Rate: 50 nl/min
Injection Site: Brain parenchyma
Brain Region: Forebrain: Hypothalamus
Injection Site Comments: Oxytocinergic neurons in paraventricular hypothalamic nucleus

View in Addgene Catalog

Subject

Species / Strain: Mouse C57/BL6J
Stock Number: Jax mouse Stock No: 024234
Age at Injection: 6 weeks old

Data Collection

Time After Injection: 2 weeks
Detection Method: Immunohistochemistry, Direct fluorescence
Expression Site: Oxytocinergic neurons in paraventricular hypothalamic nucleus
Genetic Tool Outcome: Yes