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pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 in Mouse (AAV5)

Addgene #100842-AAV5

Data Submitted By
Kengo Inada and Kazunari Miyamichi
Submission Date
August 11, 2019
Publication Date
August 11, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: GCaMP expressing neurons are visualized by anti-GFP staining (green). Coronal section. Blue, DAPI, 3V, third ventricle. Scale bar, 100 um.

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV.CAG.Flex.GCaMP6s.WPRE.SV40
Addgene #100842-AAV5

Virus & Injection

Virus

Virus Name
pAAV.CAG.Flex.GCaMP6s.WPRE.SV40
Serotype
AAV5
Source
Addgene
Catalog Number
100842-AAV5
Promoter
CAG
Cargo Type
Biosensor
Injection Titer / Dose
3.20E+13 GC/mL
Injection Volume
400 nL
Injection Rate
50 nL/min
Injector Material
Not Provided
Injection Site / Route
Paraventricular nucleus of the hypothalamus, NeuroNames ID: 387
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J
Source
Jackson Labs
Stock Number
024234
Age at Injection
6 weeks old
Time After Injection
2 weeks
Detection Method
Immunohistochemistry, Direct fluorescence
Assay & Results
Oxytocinergic neurons in paraventricular hypothalamic nucleus. GCaMP expressing neurons are visualized by anti-GFP staining (green).
Functional Testing
Yes