pZac2.1 gfaABC1D-lck-GCaMP6f in Mouse (AAV5)
Addgene #52924-AAV5
Caption: Acute cortical slice in V1 showing GCaMP+ astrocytes
Image attribution: Michelle Cahill
Virus & Injection
- Primary Virus
- Promoter
- GFAP
- Injected Titer
- 4.92E+13 GC/ml
- Injected Volume
- 30 nl/site, 8 sites per animal, 240 nl total
- Injection Rate
- 3 nl/sec
- Injection Site
- Brain parenchyma
- Brain Region
- Forebrain: Cerebral cortex (neocortex)
- Injection Site Comments
- Targeted astrocytes in V1
Subject
- Species / Strain
- Mouse C57/BL6J
- Age at Injection
- P0–3
Data Collection
- Time After Injection
- 3 weeks
- Detection Method
- Direct fluorescence
- Expression Site
- Cortical astrocytes
- Genetic Tool Outcome
- Calcium dynamics looked as expected