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pZac2.1 gfaABC1D-lck-GCaMP6f in Mouse (AAV5)

Addgene #52924-AAV5

Data Submitted By: Kira Poskanzer
Submission Date: July 29, 2019
Publication Date: July 29, 2019 (modified May 16, 2023)
Abstract: Not Provided

Acute cortical slice in V1 showing GCaMP+ astrocytes — Caption: Acute cortical slice in V1 showing GCaMP+ astrocytes
Image attribution: Michelle Cahill

Any further use of this image requires permission from the lab who submitted the data.

Vector Used: pZac2.1 gfaABC1D-lck-GCaMP6f
Addgene #52924-AAV5

Virus & Injection

Virus

Virus Name: pZac2.1 gfaABC1D-lck-GCaMP6f
Serotype: AAV5
Source: Addgene
Catalog Number: 52924-AAV5
Promoter: GFAP
Cargo Type: Biosensor
Injection Titer / Dose: 4.92E+13 GC/mL
Injection Volume: 30 nL/site, 8 sites per animal, 240 nL total
Injection Rate: 180 nL/min
Injector Material: Not Provided
Injection Site / Route: Primary visual cortex, NeuroNames ID: 2102
Additional Details: Targeted astrocytes in V1.

View in Addgene Catalog

Other Details

Species: Mouse
Strain or Cell Line: C57/BL6J
Source: Not provided
Age at Injection: P0–3
Time After Injection: 3 weeks
Detection Method: Direct fluorescence
Assay & Results: Targeted astrocytes in V1.
Functional Testing: Calcium dynamics looked as expected.