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pAAV-hSyn-hM4D(Gi)-mCherry in Mouse (AAV2)
Addgene #50475-AAV2

Coronal mouse brain section of the MEC layer 2 long range projections in dentate gyrus labeling with DREADD receptor hM3D or hM4D was taken by Leica confocal microscope under 20X. Red channel represents mCherry, and blue color is DAPI. — Caption: Coronal mouse brain section of the MEC layer 2 long range projections in dentate gyrus labeling with DREADD receptor hM3D or hM4D was taken by Leica confocal microscope under 20X. Red channel represents mCherry, and blue color is DAPI.

Data Submitted By

Ting Feng and Simon Pieraut

Submission date: July 25, 2019

Any further use of this image requires the permission of the lab.

This scientist responded to a specific request for user data and was compensated for time and input.

Vectors Used

pAAV-hSyn-hM4D(Gi)-mCherry #50475-AAV2 deposited by Bryan Roth

Virus & Injection

Primary Virus: pAAV-hSyn-hM4D(Gi)-mCherry (AAV2)
Promoter: Syn
Injected Titer: 2.00E+12 GC/ml
Injected Volume: 0.5 μl
Injection Site: Brain parenchyma
Brain Region: Medial entorhial cortex
Injection Site Comments: Medial entorhial cortex provides input to the dentate gyrus. Our goal is to target the perforate path fiber in dentate gyrus,

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Subject

Species / Strain: Mouse C57/BL6J
Age at Injection: P1 to P3

Data Collection

Time After Injection: 2 or 3 weeks
Detection Method: Direct fluorescence
Expression Site: The perforant path fiber in dentate gyrus
Genetic Tool Outcome: We used CON to active the hM3D and hM4d. We saw the c-fos expression was changed dramatically
Tips & Comments: The expression pattern looked the same for the excitatory DREADD (50474-AAV2)