Return to Addgene.org

AAV Data HubBeta

Back to AAV Data Hub

pAAV-hSyn-DIO-hM3D(Gq)-mCherry in Mouse (AAV8)
Addgene #44361-AAV8

Dreadd (hm3d-mcherry) in red (610 emission), in cholecystokinin (cck) cells in the hypothalamus of a cck-cre mouse line, epifluorescence microscope, 4x objective — Caption: Dreadd (hm3d-mcherry) in red (610 emission), in cholecystokinin (cck) cells in the hypothalamus of a cck-cre mouse line, epifluorescence microscope, 4x objective

Data Submitted By

Avishek Adhikari

Submission date: July 18, 2019

Any further use of this image requires the permission of the lab.

This scientist responded to a specific request for user data and was compensated for time and input.

Vectors Used

pAAV-hSyn-DIO-hM3D(Gq)-mCherry #44361-AAV8 deposited by Bryan Roth

Virus & Injection

Primary Virus: pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV8)
Promoter: Syn
Injected Titer: 3.00E+12 GC/ml
Injected Volume: 60 nl
Injection Rate: 0. 3 μl/min
Injection Site: Brain parenchyma
Brain Region: Forebrain: Hypothalamus
Injection Site Comments: Cholecystokinin (cck) cells in the hypothalamus of a cck-cre mouse line

View in Addgene Catalog

Subject

Species / Strain: Mouse C57/BL6J
Stock Number: JAX: 000664
Age at Injection: 2 months

Data Collection

Time After Injection: 8 weeks
Detection Method: Direct fluorescence
Expression Site: Cholecystokinin (cck) cells in the hypothalamus of a cck-cre mouse line
Genetic Tool Outcome: The DREADD receptor worked as expected upon treatment with systemic CNO injection