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AAV-FLEX-Arch-GFP in Mouse (AAV9)
Addgene #22222-AAV9

cck+ cells in hypothalamus, arch-gfp (green) is 510 nm emission channel, 5x objective — Caption: cck+ cells in hypothalamus, arch-gfp (green) is 510 nm emission channel, 5x objective

Data Submitted By

Avishek Adhikari

Submission date: July 18, 2019

Any further use of this image requires the permission of the lab.

This scientist responded to a specific request for user data and was compensated for time and input.

Vectors Used

AAV-FLEX-Arch-GFP #22222-AAV9 deposited by Edward Boyden

Virus & Injection

Primary Virus: AAV-FLEX-Arch-GFP (AAV9)
Promoter: CAG
Injected Titer: 2.00E+12 GC/ml
Injected Volume: 40 nl
Injection Rate: 0. 3 μl/min
Injection Site: Brain parenchyma
Brain Region: Forebrain: Hypothalamus
Injection Site Comments: Cholecystokinin (cck) cells in the hypothalamus of a cck-cre mouse line

View in Addgene Catalog

Subject

Species / Strain: Mouse C57/BL6J
Stock Number: JAX: 000664
Age at Injection: 2 months

Data Collection

Time After Injection: 8 weeks
Detection Method: Direct fluorescence
Expression Site: Cholecystokinin (cck) cells in the hypothalamus of a cck-cre mouse line
Genetic Tool Outcome: Green light was used to activate arch, and the behavior worked