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AAV-FLEX-Arch-GFP in Mouse (AAV9)

Addgene #22222-AAV9

Data Submitted By
Avishek Adhikari
Submission Date
July 18, 2019
Publication Date
July 18, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: cck+ cells in hypothalamus, arch-gfp (green) is 510 nm emission channel, 5x objective

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
AAV-FLEX-Arch-GFP
Addgene #22222-AAV9

Virus & Injection

Virus

Virus Name
AAV-FLEX-Arch-GFP
Serotype
AAV9
Source
Addgene
Catalog Number
22222-AAV9
Promoter
CAG
Cargo Type
Control
Injection Titer / Dose
2.00E+12 GC/ml
Injection Volume
40 nl
Injection Rate
300 nL/min
Injector Material
Not Provided
Injection Site / Route
Hypothalamus NeuroNames ID : 375
Additional Details
Cholecystokinin (cck) cells in the hypothalamus of a cck-cre mouse line;
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J
Source
Jackson Labs
Stock Number
JAX: 000664
Age at Injection
2 months
Time After Injection
8 weeks
Detection Method
Direct fluorescence
Assay & Results
Not Provided
Functional Testing
Green light was used to activate arch, and the behavior worked