pAAV_hSyn1-SIO-stGtACR2-FusionRed in Mouse (AAV1)
Addgene #105677-AAV1
- Data Submitted By
- Kevin Monk and Marshall Hussain Shuler
- Submission Date
- July 16, 2019
- Publication Date
- July 16, 2019 (modified January 30, 2024)
- Abstract
- Not provided
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV_hSyn1-SIO-stGtACR2-FusionRed
Addgene #105677-AAV1
Virus & Injection
Virus
- Virus Name
- pAAV_hSyn1-SIO-stGtACR2-FusionRed
- Serotype
- AAV1
- Source
- Addgene
- Catalog Number
- 105677-AAV1
- Promoter
- Synapsin
- Cargo Type
- Optogenetics
- Injection Titer / Dose
- 1.00E+13 GC/ml
- Injection Volume
- 202.4 nl per hemisphere
- Injection Rate
- 55 nl/min
- Injector Material
- Not Provided
- Injection Site / Route
- Primary visual cortex, NeuroNames ID: 2102
Other Details
- Species
- Mouse
- Strain or Cell Line
- B6.129P2-Pvalbtm1(cre)Arbr/J
- Source
- Jackson Labs
- Stock Number
- 17320
- Age at Injection
- 2 - 3 months
- Time After Injection
- 6 - 8 weeks following injection animals were bilaterally implanted with recording electrodes and experiments were continued over the course of 10 additional weeks.
- Detection Method
- in vivo electrophysiology couple with optogenetic stimulation
- Assay & Results
- Not Provided
- Functional Testing
- Blue light is used to activate the channel
- Tips & Comments
- The providers of this virus (Ofer Yizhar) were incredibly helpful and prompt in their replies to our questions