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pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] in Rat (AAV5)

Addgene #62723-AAV5

Data Submitted By
Benjamin Saunders
Submission Date
July 16, 2019
Publication Date
July 16, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Green=TH, Red=Chrimson-tomato
Image attribution: Amy Wolff, PhD

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]
Addgene #62723-AAV5

Virus & Injection

Virus

Virus Name
pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]
Serotype
AAV5
Source
Addgene
Catalog Number
62723-AAV5
Promoter
Synapsin
Cargo Type
Optogenetics
Injection Titer / Dose
1.00E+13 GC/ml
Injection Volume
750 nL
Injection Rate
100 nL/min
Injector Material
Not Provided
Injection Site / Route
Ventral tegmental area, NeuroNames ID: 521
Additional Details
Targeting dopamine neurons in tyrosine-hydroxylase cre rats. Injections targeted to the ventral tegmental area and substantia nigra.
View in Addgene Catalog

Other Details

Species
Rat
Strain or Cell Line
Long Evans
Source
Not provided
Age at Injection
~4 months
Time After Injection
5 weeks
Detection Method
Immunohistochemistry, Direct fluorescence
Assay & Results
Dopaminergic/TH+ cells, as expected. The genetic targeting worked as expected.