pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] in Rat (AAV5)
Addgene #62723-AAV5

Green=TH, Red=Chrimson-tomato

Caption: Green=TH, Red=Chrimson-tomato

Image attribution: Amy Wolff, PhD

Virus & Injection

Primary Virus
Injected Titer
1.00E+13 GC/ml
Injected Volume
750 nl
Injection Rate
0. 1 μl/min
Injection Site
Directly into brain tissue
Brain Region
Midbrain: Tegmentum: Substantia nigra
Injection Site Comments
Targeting dopamine neurons in tyrosine-hydroxylase cre rats. Injections targeted to the ventral tegmental area and substantia nigra
View in Addgene Catalog


Species / Strain
Rat Long Evans
Age at Injection
~4 months

Data Collection

Time After Injection
5 weeks
Detection Method
Immunohistochemistry, Direct fluorescence
Expression Site
Dopaminergic/TH+ cells, as expected
Genetic Tool Outcome
Yes, the genetic targeting worked as expected