Skip to main content

pAAV-hSyn-DIO-mCherry in Mouse (AAV5)

Addgene #50459-AAV5

Data Submitted By
Henry Hallock and Keri Martinowich
Submission Date
July 11, 2019
Publication Date
July 11, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Imaged in the ventral hippocampus with a 10x objective on a confocal microscope, laser wavelength of 555 nm.

Any further use of this image requires permission from the lab who submitted the data.

Vectors Used
pAAV-hSyn-DIO-mCherry
Addgene #50459-AAV5
pENN.AAV.hSyn.Cre.WPRE.hGH
Addgene #105553-AAVrg

Virus & Injection

Virus 1

Virus Name
pAAV-hSyn-DIO-mCherry
Serotype
AAV5
Source
Addgene
Catalog Number
50459-AAV5
Promoter
Synapsin
Cargo Type
Control
Injection Titer / Dose
4.10E+12 GC/ml
Injection Volume
600 nl
Injection Rate
200 nl/min
Injector Material
Not Provided
Injection Site / Route
CA1 field NeuroNames ID : 182
Additional Details
Injected into the ventral hippocampus to target CA1 cells projecting to the medial prefrontal cortex; Forebrain: Hippocampus; Ventral hippocampal CA1 pyramidal neurons projecting to the mPFC
View in Addgene Catalog

Virus 2

Virus Name
pENN.AAV.hSyn.Cre.WPRE.hGH
Serotype
AAV Retrograde
Source
Addgene
Catalog Number
105553-AAVrg
Promoter
Synapsin
Cargo Type
Recombinase
Injection Titer / Dose
1.20E+13 GC/ml
Injection Volume
600 nl
Injection Rate
200 nl/min
Injector Material
Not Provided
Injection Site / Route
Prefrontal Cortex, Neuronames: 1351 Prelimbic area, (area 25 of Brodmann (guenon), Neuronames: 1029
Additional Details
Prelimbic subregion of the medial PFC; Forebrain: Cerebral cortex (neocortex)
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J
Source
Not provided
Other Information
Mutant mice with disruption of BDNF from promoter IV (bred in-house: Maynard et al., https://www.nature.com/articles/npp2015349).
Age at Injection
3 months
Time After Injection
6 weeks
Detection Method
Direct fluorescence
Assay & Results
Not Provided
Other Information
No toxicity/inflammation
Tips & Comments
I would use this again for this experiment. Worked well