pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA in Mouse (AAV1)
Addgene #50943-AAV1
Caption: None provided
Virus & Injection
- Primary Virus
- Promoter
- Syn
- Injected Titer
- 1.00E+10 GC/ml
- Injected Volume
- 4 μl (2 μl per hemisphere)
- Injection Rate
- Injections were done by hand
- Injection Site
- Cerebrospinal fluid (CSF): Intracerebroventricular (ICV)
- Brain Region
- We target the ventricles for widepsread transduction
- Injection Site Comments
- Neurons were targeted
Subject
- Species / Strain
- Mouse B6SJL F1 hybrid
- Stock Number
- 100012
- Age at Injection
- P0 (first 24 hrs after birth)
Data Collection
- Time After Injection
- 1 month to 6 months
- Detection Method
- Immunohistochemistry, Direct fluorescence
- Expression Site
- Neurons in forebrain- cortex, hippocampus, some mid brain
- Genetic Tool Outcome
- The GCaMP6 activated spontaneously in some neurons, suggesting it worked effectively. The mRuby was visible in cell bodies and neurites, marking transduced cells
- Other Information
- No evidence of toxicity or other adverse effects. Expression persisted through life of animal, up to at least 16 months