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pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA in Mouse (AAV1)

Addgene #50943-AAV1

Data Submitted By
Katherine Sadleir and Robert Vassar
Submission Date
July 11, 2019
Publication Date
July 11, 2019 (modified May 16, 2023)
Abstract
Not provided

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Vector Used
pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA
Addgene #50943-AAV1

Virus & Injection

Virus

Virus Name
pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA
Serotype
AAV1
Source
Addgene
Catalog Number
50943-AAV1
Promoter
Synapsin
Cargo Type
Biosensor
Injection Titer / Dose
1.00E+10 GC/ml
Injection Volume
4 μl (2 μl per hemisphere)
Injection Rate
Injections were done by hand
Injector Material
Not provided
Injection Site / Route
Intracerebroventricular (ICV). NeuroNames ID : 2497
Additional Details
Neurons were targeted; We target the ventricles for widepsread transduction; Neurons in forebrain- cortex, hippocampus, some mid brain
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
B6SJL F1 hybrid
Source
Not provided
Stock Number
100012
Age at Injection
P0 (first 24 hrs after birth)
Time After Injection
1 month to 6 months
Detection Method
Immunohistochemistry, Direct fluorescence
Assay & Results
Not provided
Functional Testing
The GCaMP6 activated spontaneously in some neurons, suggesting it worked effectively. The mRuby was visible in cell bodies and neurites, marking transduced cells
Other Information
No evidence of toxicity or other adverse effects. Expression persisted through life of animal, up to at least 16 months