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pAAV.hSyn1.Twitch2B.WPRE.SV40 in Mouse (AAV1)

Addgene #100040-AAV1

Data Submitted By
Katherine Sadleir and Robert Vassar
Submission Date
July 11, 2019
Publication Date
July 11, 2019 (modified May 16, 2023)
Abstract
Not provided
live slice on Nikon A1RMP+ Multiphoton with 25x objective, CFP/YFP filter was used to detect Twitch fluorescence (blue and green respectively in the image)
Caption: live slice on Nikon A1RMP+ Multiphoton with 25x objective, CFP/YFP filter was used to detect Twitch fluorescence (blue and green respectively in the image)
Image attribution: Katherine Sadleir

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV.hSyn1.Twitch2B.WPRE.SV40
Addgene #100040-AAV1

Virus & Injection

Virus

Virus Name
pAAV.hSyn1.Twitch2B.WPRE.SV40
Serotype
AAV1
Source
Addgene
Catalog Number
100040-AAV1
Promoter
Synapsin
Cargo Type
Biosensor
Injection Titer / Dose
1.00E+10 GC/ml
Injection Volume
4 μl (2 μl per hemisphere)
Injection Rate
Done by hand
Injector Material
Not provided
Injection Site / Route
Intracerebroventricular (ICV). NeuroNames ID : 2497
Additional Details
Neurons were targeted; We target the ventricles; Neurons of the forebrain, cortex, hippocampus some midbrain as well
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
B6SJL F1 hybrid
Stock Number
100012
Age at Injection
P0 (first 24 hrs after birth)
Time After Injection
1 month to 6 months
Detection Method
Direct fluorescence
Functional Testing
This is a ratiometric calcium sensor- different cells showed different ratios, responded to calcium changes
Other Information
No signs of toxicity or damage, expression lasted lifespan of animal (up to 16 months at least)