Skip to main content

pENN.AAV.hSyn.Cre.WPRE.hGH in Mouse (AAV1)

Addgene #105553-AAV1

Data Submitted By
Farhan Ali and Alex Kwan
Submission Date
July 10, 2019
Publication Date
July 10, 2019 (modified May 16, 2023)
Abstract
Not provided
Caption: in vivo two-photon image. Stitched from individual images that traced a single neuron's soma and its dendrites for ~100 microns as labeled by the combination of sparse Cre expression and Cre-dependent GCaMP6s

Any further use of this image requires permission from the lab who submitted the data.

Vectors Used
pENN.AAV.hSyn.Cre.WPRE.hGH
Addgene #105553-AAV1
pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA
Addgene #68720-AAV1

Virus & Injection

Virus 1

Virus Name
pENN.AAV.hSyn.Cre.WPRE.hGH
Serotype
AAV1
Source
Addgene
Catalog Number
105553-AAV1
Promoter
Synapsin
Cargo Type
Recombinase
Injection Titer / Dose
1.00E+08 GC/ml
Injection Volume
200 nl
Injection Rate
40 nl/min
Injector Material
Not provided
Injection Site / Route
Dorsal anterior cingulate cortex/medial secondary motor cortex of mouse. NeuroNames ID : 1062
Additional Details
Injection site: dorsal anterior cingulate cortex/medial secondary motor cortex of mouse Cell type: any neuron; Forebrain: Cerebral cortex (neocortex); Dorsal anterior cingulate cortex/medial secondary motor cortex of mouse. Neuronal cell class
View in Addgene Catalog

Virus 2

Virus Name
pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA
Serotype
AAV1
Source
Addgene
Catalog Number
68720-AAV1
Promoter
Synapsin
Injection Titer / Dose
1.00E+13 GC/ml
Injection Volume
200 nl
Injection Rate
40 nl/min
Injector Material
Not provided
Injection Site / Route
Dorsal anterior cingulate cortex/medial secondary motor cortex of mouse. NeuroNames ID : 1062
Additional Details
Injection site: dorsal anterior cingulate cortex/medial secondary motor cortex of mouse. Cell type: any neuron type; Forebrain: Cerebral cortex (neocortex)
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J
Source
Not provided
Age at Injection
8-20 weeks
Time After Injection
5 weeks
Detection Method
Direct fluorescence
Assay & Results
Not provided
Other Information
This experiment targeted very sparse expression of GCaMP6s by combining Cre-dependent GCaMP6s expression with injection of very low titer of virus carrying Cre recombinase