pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 in Rat (AAV5)
Addgene #100842-AAV5
Caption: Blue - DAPI Red - TH Cre Green - GFP objective: 10X microscope: Zeiss 700 confocal
Image attribution: Jasmin Multani
Virus & Injection
- Primary Virus
- Promoter
- CAG
- Injected Titer
- 3.20E+13 GC/ml
- Injected Volume
- two 1 μl injections
- Injection Rate
- 75 nl/min
- Injection Site
- Brain parenchyma
- Brain Region
- Hindbrain: Pons
- Injection Site Comments
- Noradrenergic cells of the locus coeruleus
Subject
- Species / Strain
- Rat LE-Tg(TH-Cre)3.1Deis
- Stock Number
- RRRC#: 659
- Age at Injection
- 3-4 months
Data Collection
- Time After Injection
- Tested 1 week to 8 weeks after injection
- Detection Method
- Immunohistochemistry, fiber photometry
- Expression Site
- Limited expression in noradrenergic cells of the locus coeruleus
- Genetic Tool Outcome
- I was able to get somewhat reliable fiber photometry signal
- Other Information
- No
- Tips & Comments
- I tried multiple capsids and promoters and found that this combination is the best