Return to AAV Results

pAAV-Ef1a-DIO EYFP in Rat (AAV5)

Addgene #27056-AAV5

Data Submitted By: Erin Glennon and Robert Froemke
Submission Date: July 09, 2019
Publication Date: July 09, 2019 (modified May 16, 2023)
Abstract: Not Provided

Blue - DAPI Red - TH Cre Green - YFP objective: 10X microscope: Zeiss 700 Confocal — Caption: Blue - DAPI Red - TH Cre Green - YFP objective: 10X microscope: Zeiss 700 Confocal
Image attribution: Jasmin Multani

Any further use of this image requires permission from the lab who submitted the data.

Vector Used: pAAV-Ef1a-DIO EYFP
Addgene #27056-AAV5

Virus & Injection

Virus

Virus Name: pAAV-Ef1a-DIO EYFP
Serotype: AAV5
Source: Addgene
Catalog Number: 27056-AAV5
Promoter: EF1a
Cargo Type: Control
Injection Titer / Dose: 3.30E+13 GC/mL
Injection Volume: two 1 μl injections
Injection Rate: 75 nL/sec
Injector Material: Not Provided
Injection Site / Route: Locus ceruleus, NeuroNames ID: 583

View in Addgene Catalog

Other Details

Species: Rat
Strain or Cell Line: LE-Tg(TH-Cre)3.1Deis
Source: Rat Resource & Research Center
Stock Number: 00659
Age at Injection: 3-4 months
Time After Injection: 2 weeks
Detection Method: Immunohistochemistry
Assay & Results: Noradrenergic cells of the locus coeruleus.
Tips & Comments: AAV5 works well for this cell type.