AAV-hSyn1-GCaMP6s-P2A-nls-dTomato in Mouse (AAV Retrograde)
Addgene #51085-AAVrg
Caption: Co-localization of GCaMP6-derived GFP and tdTomato in ventral hippocampal CA1. Still shots taken with a confocal microscope.
Virus & Injection
- Primary Virus
- Promoter
- Syn
- Injected Titer
- 7.69E+12 GC/ml
- Injected Volume
- 600 nl
- Injection Rate
- 200 nl/min
- Injection Site
- Brain parenchyma
- Brain Region
- Forebrain: Cerebral cortex (neocortex)
- Injection Site Comments
- Injected into prelimbic subregion of medial PFC. Targeting ventral hippocampal cells projecting to medial PFC
Subject
- Species / Strain
- Mouse C57/BL6J
- Stock Number
- JAX: 000664
- Age at Injection
- 3 months
Data Collection
- Time After Injection
- 4 weeks
- Detection Method
- Direct fluorescence
- Expression Site
- Ventral hippocampal CA1 cells
- Other Information
- No toxicity/inflammation
- Tips & Comments
- Although GFP and tdTomato expression was seen in the ventral hippocampus, fluorescence was not dynamic - so didn't seem to work well for this cell class. We imaged in ex vivo hippocampal slices on a confocal microscope with a spinning disk