pAAV-CAG-tdTomato (codon diversified) in Mouse (PHP.eB)
Addgene #59462-PHPeB
Caption: tdTomato in green; NeuroTrace 640 in magenta. Imaged on Olympus Vs120 slide scanner with 10x objective, 60 ms exposure
Virus & Injection
- Primary Virus
- Promoter
- CAG
- Injected Titer
- 1.00E+12 GC/ml
- Injected Volume
- 100 μl
- Injection Rate
- Via 0. 3 mL insulin syringe
- Injection Site
- Intravenous (retro-orbital or tail vein)
- Injection Site Comments
- Retro-orbital injection to target cells throughout the brain with the PHP. EB serotype
Subject
- Species / Strain
- Mouse C57/BL6J
- Stock Number
- JAX: 000664
- Age at Injection
- 8 weeks
Data Collection
- Time After Injection
- 3 weeks
- Detection Method
- Direct fluorescence
- Expression Site
- Widespread infection throughout the brain. In both excitatory pyramidal neurons, as well as glia. Density was fairly uniform across cortical regions
- Other Information
- I didn't quantify it, but the percentage of glia infected varied across mice. I didn't see any signs of toxicity (e. G. No signs of blebbing in axons), but I also only had tdTomato
- Tips & Comments
- PHP. EB + CAG seems like a great combination for transducing a large numbers of neurons across the brain