AAV pmSyn1-EBFP-Cre in Rat (AAV Retrograde)
Addgene #51507-AAVrg
- Data Submitted By
- Christina Ruiz and Stephen V Mahler
- Submission Date
- June 25, 2019
- Publication Date
- June 25, 2019 (modified May 16, 2023)
- Abstract
- Not provided
Image attribution: Mitchell Farrell
Image attribution: Mitchell Farell
Any further use of this image requires permission from the lab who submitted the data.
- Vectors Used
-
AAV pmSyn1-EBFP-Cre
Addgene #51507-AAVrg -
pAAV-hSyn-DIO-hM4D(Gi)-mCherry
Addgene #44362-AAV2
Virus & Injection
Virus 1
- Virus Name
- AAV pmSyn1-EBFP-Cre
- Serotype
- AAV Retrograde
- Source
- Addgene
- Catalog Number
- 51507-AAVrg
- Promoter
- Synapsin
- Cargo Type
- Recombinase
- Injection Titer / Dose
- 6.00E+12 GC/ml
- Injection Volume
- 300 nl
- Injection Rate
- 600 nl/min
- Injector Material
- Not provided
- Injection Site / Route
- Ventral Pallidum, NeuroNames ID : 1605
- Additional Details
- Targeting neurons in Ventral Pallidum with AAVrg and Nucleus Accumbens cells that project to VP with DIO-hM4DGi; Ventral pallidum; AAVrg-cre was visualized in VP cells expressing synapsin. DIO-hM4D(Gi) was visualized in cells of NAc that project to VP
Virus 2
- Virus Name
- pAAV-hSyn-DIO-hM4D(Gi)-mCherry
- Serotype
- AAV2
- Source
- Addgene
- Catalog Number
- 44362-AAV2
- Promoter
- Synapsin
- Injection Titer / Dose
- 5.00E+12 GC/ml
- Injection Volume
- 300 nl
- Injection Rate
- 600 nl/min
- Injector Material
- Not provided
- Injection Site / Route
- Nucleus Accumbens
- Additional Details
- Targeting neurons in nucleus accumbens projecting to ventral pallidum. Injected 15 min prior to previous injection; Nucleus accumbens
Other Details
- Species
- Rat
- Strain or Cell Line
- Wild-type Long Evans
- Stock Number
- Envigo
- Age at Injection
- postnatal day 60-80
- Time After Injection
- 3 weeks
- Detection Method
- Immunofluorescence for mCherry and GFP
- Other Information
- No toxicity or inflammation seen
- Tips & Comments
- We waited 5 minutes before removing the injection pipette from the injection site