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pAAV-CaMKIIa-eNpHR 3.0-EYFP in Mouse (AAV1)

Addgene #26971-AAV1

Data Submitted By: Dong Wang
Submission Date: June 26, 2019
Publication Date: June 26, 2019 (modified May 16, 2023)
Abstract: Not Provided

Taken with Leica DM 5500B. Fluorescence in the dorsal hippocampus (injection site) and granular retrospenial cortex (RSC) layer 2/3 (projection site). The track of an optic fiber implantation into the dorsal RSC is visible. — Caption: Taken with Leica DM 5500B. Fluorescence in the dorsal hippocampus (injection site) and granular retrospenial cortex (RSC) layer 2/3 (projection site). The track of an optic fiber implantation into the dorsal RSC is visible.

Any further use of this image requires permission from the lab who submitted the data.

Vector Used: pAAV-CaMKIIa-eNpHR 3.0-EYFP
Addgene #26971-AAV1

Virus & Injection

Virus

Virus Name: pAAV-CaMKIIa-eNpHR 3.0-EYFP
Serotype: AAV1
Source: Addgene
Catalog Number: 26971-AAV1
Promoter: CaMKII
Cargo Type: Optogenetics
Injection Titer / Dose: 1.00E+13 GC/ml
Injection Volume: 200 nl
Injection Rate: 50 nl/min
Injector Material: Not Provided
Injection Site / Route: CA1 field, NeuroNames ID: 183
Additional Details: Targeting CA1 pyramidal neurons, subiculum, dentate gyrus.

View in Addgene Catalog

Other Details

Species: Mouse
Strain or Cell Line: C57/BL6J
Source: Not provided
Age at Injection: 10-13 weeks
Time After Injection: 4 weeks
Detection Method: Direct fluorescence, in vivo electrophysiology
Assay & Results: Not Provided
Functional Testing: Yes, laser stimulation activates infected neurons.
Tips & Comments: Inject less volume, such as 100 nl.