pAAV-hSyn-hM4D(Gi)-mCherry in Rat (AAV Retrograde)
Addgene #50475-AAVrg
Caption: Zeiss LSM 710 Confocal, 10x in prelimbic 568 channel for DsRed staining (Living Colors rabbit polyclonal antibody)
Virus & Injection
- Primary Virus
- Promoter
- Syn
- Injected Titer
- 1.00E+13 GC/ml
- Injected Volume
- two 750 nl injections
- Injection Rate
- 150 nl/min
- Injection Site
- Brain parenchyma
- Brain Region
- Hindbrain: Pons
- Injection Site Comments
- Pyramidal tract cortical neurons
Subject
- Species / Strain
- Rat Sprague Dawley
- Age at Injection
- ~2-3 months old
Data Collection
- Time After Injection
- 2 weeks
- Detection Method
- Immunohistochemistry
- Expression Site
- Pyramidal tract neurons in motor, sensory, cingulate, and prelimbic cortices. Some expression in ventroorbital cortex and infralimbic cortex as well
- Genetic Tool Outcome
- Yes, there was CNO-induced behavioral changes seen in animals expressing Gi DREADD versus vehicle controls
- Tips & Comments
- This injection can be very bloody. It may be smart to go at an angle for pons targeting