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pAAV-CaMKIIa-hM3D(Gq)-mCherry in Rat (AAV5)

Addgene #50476-AAV5

Data Submitted By: Michael Baratta
Submission Date: June 20, 2019
Publication Date: June 20, 2019 (modified May 16, 2023)
Abstract: Not Provided

Red = hM3Dq-mCherry expression Green = Fos protein (Alexa Fluor 488) — Caption: Red = hM3Dq-mCherry expression Green = Fos protein (Alexa Fluor 488)

Any further use of this image requires permission from the lab who submitted the data.

Vector Used: pAAV-CaMKIIa-hM3D(Gq)-mCherry
Addgene #50476-AAV5

Virus & Injection

Virus

Virus Name: pAAV-CaMKIIa-hM3D(Gq)-mCherry
Serotype: AAV5
Source: Addgene
Catalog Number: 50476-AAV5
Promoter: CaMKII
Cargo Type: Chemogenetics
Injection Titer / Dose: 3.10E+13 GC/mL
Injection Volume: 500 nL
Injection Rate: 100 nL/min
Injector Material: Not Provided
Injection Site / Route: Medial group of the dorsal thalamus, NeuroNames ID: 2684

View in Addgene Catalog

Other Details

Species: Rat
Strain or Cell Line: Sprague-Dawley
Source: Not provided
Age at Injection: 9-11 weeks
Sex: Male
Time After Injection: 3 weeks
Detection Method: Direct fluorescence
Assay & Results: CaMKIIa-positive cells in Medial Dorsal Thalamus.
Functional Testing: Yes, CNO (3 mg/kg; i. P. ) induced Fos protein 90 minutes post injection.