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pAAV-hSyn-DIO-mCherry in Rat (AAV8)

Addgene #50459-AAV8

Data Submitted By
Michael Baratta
Submission Date
June 20, 2019
Publication Date
June 20, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Prelimbic neurons that project to dorsal raphe nucleus, Zeiss 510 Confocal Laser Scanning Microscope, 543 nm laser, 20X objective

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV-hSyn-DIO-mCherry
Addgene #50459-AAV8

Virus & Injection

Virus

Virus Name
pAAV-hSyn-DIO-mCherry
Serotype
AAV8
Source
Addgene
Catalog Number
50459-AAV8
Promoter
Synapsin
Cargo Type
Control
Injection Titer / Dose
2.10E+13 GC/mL
Injection Volume
1 μL per hemisphere
Injection Rate
100 nL/min
Injector Material
Not Provided
Injection Site / Route
Prelimbic area, NeuroNames ID: 1078
View in Addgene Catalog

Other Details

Species
Rat
Strain or Cell Line
Sprague Dawley
Source
Not provided
Age at Injection
9-11 weeks
Time After Injection
3 weeks
Detection Method
Direct fluorescence
Assay & Results
Prelimbic region of the medial prefrontal cortex. Prelimbic neurons that project to the dorsal raphe nucleus. Yes, mCherry expression was restricted to dorsal raphe-projecting prelimbic neurons (Cre-positive). Expression was robust.
Other Information
No toxicity was observed, minimal damage surrounding injection site.