pAAV-CAG-tdTomato (codon diversified) in Mouse (AAV Retrograde)
Addgene #59462-AAVrg
Caption: Tdtomato flourescence observed in a 300 um coronal brain slice on an upright Olympus BX53-series microscope.
Virus & Injection
- Primary Virus
- Promoter
- CAG
- Injected Titer
- 1.00E+13 GC/ml
- Injected Volume
- 200 nl
- Injection Rate
- 100 nl/min
- Injection Site
- Brain parenchyma
- Brain Region
- Forebrain: Amygdala
- Injection Site Comments
- Aiming to target pyramidal cells in the basolateral amygdala. We also targeted medium spiny neurons in the anterior nucleus accumbens, at the interface between the core and shell. The aim was to record from pyramidal cells in deep layer prelimbic prefrontal cortex, which we found with extensive tdTomato expression after this procedure
Subject
- Species / Strain
- Mouse C57/BL6J
- Stock Number
- JAX: 000664
- Age at Injection
- 4-5 weeks
Data Collection
- Time After Injection
- 1 week
- Detection Method
- Direct fluorescence
- Expression Site
- Neurons within the injection site as well as pyramidal cells throughout the prefrontal cortex
- Genetic Tool Outcome
- Not applicable
- Other Information
- Worked wonderfully
- Tips & Comments
- No. Very straightforward