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pAAV-hSyn-DIO-hM4D(Gi)-mCherry in Rat (AAV8)

Addgene #44362-AAV8

Data Submitted By
Elizabeth Crummy and Susan Ferguson
Submission Date
June 20, 2019
Publication Date
June 20, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Alex fluor 568 antibody 568 channel Zeiss LSM 710 confocal on 10x objective

Any further use of this image requires permission from the lab who submitted the data.

Vectors Used
pAAV-hSyn-DIO-hM4D(Gi)-mCherry
Addgene #44362-AAV8
CAV2-CRE

Virus & Injection

Virus 1

Virus Name
pAAV-hSyn-DIO-hM4D(Gi)-mCherry
Serotype
AAV8
Source
Addgene
Catalog Number
44362-AAV8
Promoter
Synapsin
Cargo Type
Chemogenetics
Injection Titer / Dose
2.00E+13 GC/mL
Injection Volume
750 nL
Injection Rate
150 nL/min
Injector Material
Not Provided
Injection Site / Route
Orbital gyri, NeuroNames ID: 91
Additional Details
Pyramidal tract neurons in the orbitofrontal cortex (CRE injected in the pons).
View in Addgene Catalog

Virus 2

Virus Name
CAV2-CRE
Serotype
N/A
Source
Unknown
Catalog Number
N/A
Promoter
Other
Other Promoter
Not Provided.
Injection Titer / Dose
Not Provided
Injection Volume
1 μL injections
Injection Rate
200 nL/min
Injector Material
Not Provided
Injection Site / Route
Pons, NeuroNames ID: 547
Additional Details
Pyramidal tract neurons. Surgeries for both CAV and AAV injections were performed the same day. The order of injections was alternated.

Other Details

Species
Rat
Strain or Cell Line
Sprague Dawley; Long Evans
Source
Not provided
Age at Injection
~2-3 months
Time After Injection
2 weeks
Detection Method
Immunohistochemistry
Assay & Results
Around lateral and ventral orbital cortex.
Functional Testing
CNO (5 mg/kg) was used for transient inhibition of pyramidal tract neurons. Within subject controls were utilized. Expression was successfully verified with living colors dsRed rabbit polyclonal antibody.