pAAV-hSyn-hM3D(Gq)-mCherry in Rat (AAV2)
Addgene #50474-AAV2
- Data Submitted By
- David Bortz and Anthony A Grace
- Submission Date
- June 19, 2019
- Publication Date
- June 19, 2019 (modified May 16, 2023)
- Abstract
- Not Reported
Image attribution: David Bortz
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-hSyn-hM3D(Gq)-mCherry
Addgene #50474-AAV2
Virus & Injection
Virus
- Virus Name
- pAAV-hSyn-hM3D(Gq)-mCherry
- Serotype
- AAV2
- Source
- Addgene
- Catalog Number
- 50474-AAV2
- Promoter
- Synapsin
- Cargo Type
- Chemogenetics
- Injection Titer / Dose
- 3.00E+12 GC/ml
- Injection Volume
- 8 μL- 1 injection
- Injection Rate
- 100 nl/min
- Injector Material
- Not Provided
- Injection Site / Route
- Medial septal nucleus NeuroNames ID : 262
- Additional Details
- I was trying to hit the anterior-posterior extent of the medial septum without getting into lateral septum or other parts of the basal forebrain, Medial septum.
Other Details
- Species
- Rat
- Strain or Cell Line
- Sprague Dawley, male from Envigo
- Source
- Not provided
- Age at Injection
- Young adult (weight 300-325 grams)
- Time After Injection
- 8 weeks for cell body expression, 12 weeks for terminal expression.
- Detection Method
- Immunohistochemistry
- Assay & Results
- Good expression on cell bodies within the medial septum as well as terminals in terminating regions. Cell type unknown.
- Other Information
- No toxicity detected
- Tips & Comments
- The one tip I would offer would be to leave the syringe at the place of infusion for several minutes following the infusion, and then raise it very slowly. This procedure has been effective for us in minimizing "dragging" the virus up with the syringe and out of the region of interest