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pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA in Mouse (AAV9)

Addgene #20298-AAV9

Data Submitted By
Liang Chen and Shaoyu Ge
Submission Date
June 19, 2019
Publication Date
June 19, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Green channel, Olympus confocal
Image attribution: Liang

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
Addgene #20298-AAV9

Virus & Injection

Virus

Virus Name
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
Serotype
AAV9
Source
Addgene
Catalog Number
20298-AAV9
Promoter
EF1a
Cargo Type
Optogenetics
Injection Titer / Dose
1.00E+13 GC/ml
Injection Volume
300 nl
Injection Rate
100 nL/min
Injector Material
Not Provided
Injection Site / Route
Zona incerta, NeuroNames ID: 438
Additional Details
We want to target ZI PV cells.
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
B6 PVcre
Source
Jackson Labs
Stock Number
JAX: 017320
Age at Injection
8 weeks
Time After Injection
3 weeks
Detection Method
Direct fluorescence
Assay & Results
We can see few glia cells surrounding the injection site.
Functional Testing
ChR2, we want to use light to activation special cells.
Tips & Comments
Do not dilute the virus.