pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA in Mouse (AAV9)
Addgene #20298-AAV9

Green channel, Olympus confocal

Caption: Green channel, Olympus confocal

Image attribution: Liang

Virus & Injection

Primary Virus
Injected Titer
1.00E+13 GC/ml
Injected Volume
300 nl
Injection Rate
0. 1 μl/min
Injection Site
Zona incerta
Brain Region
Forebrain: Subthalamus: Zona incerta
Injection Site Comments
We want to target ZI PV cells
View in Addgene Catalog


Species / Strain
Mouse B6 PVcre
Stock Number
JAX: 017320
Age at Injection
8 weeks

Data Collection

Time After Injection
3 weeks
Detection Method
Direct fluorescence
Expression Site
ZI PV cells
Genetic Tool Outcome
ChR2, we want to use light to activation special cells
Other Information
We can see few glia cells surrounding the injection site
Tips & Comments
Do not dilute the virus