pAAV-hSyn-hM3D(Gq)-mCherry in Rat (AAV8)
Addgene #50474-AAV8
Caption: Gq-DREADD expression in internal capsule-adjacent areas, eg, GP, EPN, Reticular Thalamus.
Virus & Injection
- Primary Virus
- Promoter
- Syn
- Injected Titer
- 2.00E+12 GC/ml
- Injected Volume
- 450nl
- Injection Rate
- 1nl/sec
- Injection Site
- Brain parenchyma
- Brain Region
- Forebrain: Basal Ganglia: Globus pallidus internal or entopeduncular nucleus
Subject
- Species / Strain
- Rat Long-Evans
- Age at Injection
- ~100 days old
Data Collection
- Time After Injection
- 4 weeks
- Detection Method
- Immunohistochemistry
- Genetic Tool Outcome
- Neurons expressing the excitatory DREADD exhibited robust induction of cFos following systemic injection of DREADD ligand, Deschloroclozapine